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Vector Laboratories species specific rabbit conjugated polymer
Species Specific Rabbit Conjugated Polymer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cd45ro-percp (clone uchl1; cat. 560607)
10 to 14 days post transduction, flow cytometric analysis was used to determine (A) Frequency of CD8+ and CD4+ T cells subsets (n=3, non-significant (NS), one-way ANOVA); (B) Frequency of naïve <t>(CD45RO−</t> CCR7+), effector memory (CD45RO+ CCR7−), central memory (CD45RO+ CCR7+), and EMRA (CD45RO− CCR7−) T cells (n=6; naïve and EMRA T cells: NT vs CD19-ENG +/− costimulatory molecules: p≤0.001, one-way ANOVA); Frequency of Tim3, PD-1, and LAG3 expression on (C) CD4+ cells and (D) CD8+ cells (n=3, NS, one-way ANOVA).
Cd45ro Percp (Clone Uchl1; Cat. 560607), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10 to 14 days post transduction, flow cytometric analysis was used to determine (A) Frequency of CD8+ and CD4+ T cells subsets (n=3, non-significant (NS), one-way ANOVA); (B) Frequency of naïve <t>(CD45RO−</t> CCR7+), effector memory (CD45RO+ CCR7−), central memory (CD45RO+ CCR7+), and EMRA (CD45RO− CCR7−) T cells (n=6; naïve and EMRA T cells: NT vs CD19-ENG +/− costimulatory molecules: p≤0.001, one-way ANOVA); Frequency of Tim3, PD-1, and LAG3 expression on (C) CD4+ cells and (D) CD8+ cells (n=3, NS, one-way ANOVA).
Glass Bottom Central Wells, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellvis Inc 8 well chambered coverglass system
10 to 14 days post transduction, flow cytometric analysis was used to determine (A) Frequency of CD8+ and CD4+ T cells subsets (n=3, non-significant (NS), one-way ANOVA); (B) Frequency of naïve <t>(CD45RO−</t> CCR7+), effector memory (CD45RO+ CCR7−), central memory (CD45RO+ CCR7+), and EMRA (CD45RO− CCR7−) T cells (n=6; naïve and EMRA T cells: NT vs CD19-ENG +/− costimulatory molecules: p≤0.001, one-way ANOVA); Frequency of Tim3, PD-1, and LAG3 expression on (C) CD4+ cells and (D) CD8+ cells (n=3, NS, one-way ANOVA).
8 Well Chambered Coverglass System, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellvis Inc 6 well glass bottom plate
10 to 14 days post transduction, flow cytometric analysis was used to determine (A) Frequency of CD8+ and CD4+ T cells subsets (n=3, non-significant (NS), one-way ANOVA); (B) Frequency of naïve <t>(CD45RO−</t> CCR7+), effector memory (CD45RO+ CCR7−), central memory (CD45RO+ CCR7+), and EMRA (CD45RO− CCR7−) T cells (n=6; naïve and EMRA T cells: NT vs CD19-ENG +/− costimulatory molecules: p≤0.001, one-way ANOVA); Frequency of Tim3, PD-1, and LAG3 expression on (C) CD4+ cells and (D) CD8+ cells (n=3, NS, one-way ANOVA).
6 Well Glass Bottom Plate, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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System Biosciences Inc mc easytm minicircle production system
Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and <t>minicircle</t> constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18
Mc Easytm Minicircle Production System, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pickering Laboratories o phthalaldehyde
Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and <t>minicircle</t> constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18
O Phthalaldehyde, supplied by Pickering Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson bd vacutainer edta
Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and <t>minicircle</t> constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18
Bd Vacutainer Edta, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Cellvis Inc petri dishes
Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and <t>minicircle</t> constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18
Petri Dishes, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellvis Inc lids
Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and <t>minicircle</t> constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18
Lids, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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101Bio mouse tail dna extraction kit
Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and <t>minicircle</t> constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18
Mouse Tail Dna Extraction Kit, supplied by 101Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImmuneTech Inc allergen-specific ige (alternaria species)
Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and <t>minicircle</t> constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18
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Image Search Results


10 to 14 days post transduction, flow cytometric analysis was used to determine (A) Frequency of CD8+ and CD4+ T cells subsets (n=3, non-significant (NS), one-way ANOVA); (B) Frequency of naïve (CD45RO− CCR7+), effector memory (CD45RO+ CCR7−), central memory (CD45RO+ CCR7+), and EMRA (CD45RO− CCR7−) T cells (n=6; naïve and EMRA T cells: NT vs CD19-ENG +/− costimulatory molecules: p≤0.001, one-way ANOVA); Frequency of Tim3, PD-1, and LAG3 expression on (C) CD4+ cells and (D) CD8+ cells (n=3, NS, one-way ANOVA).

Journal: Cancer immunology research

Article Title: CD28 and 41BB costimulation enhance the effector function of CD19-specific engager T cells

doi: 10.1158/2326-6066.CIR-17-0171

Figure Lengend Snippet: 10 to 14 days post transduction, flow cytometric analysis was used to determine (A) Frequency of CD8+ and CD4+ T cells subsets (n=3, non-significant (NS), one-way ANOVA); (B) Frequency of naïve (CD45RO− CCR7+), effector memory (CD45RO+ CCR7−), central memory (CD45RO+ CCR7+), and EMRA (CD45RO− CCR7−) T cells (n=6; naïve and EMRA T cells: NT vs CD19-ENG +/− costimulatory molecules: p≤0.001, one-way ANOVA); Frequency of Tim3, PD-1, and LAG3 expression on (C) CD4+ cells and (D) CD8+ cells (n=3, NS, one-way ANOVA).

Article Snippet: Flow cytometric analysis Monoclonal antibodies (mAb) for the following markers were used for fluorescence activated cell sorting (FACS) analysis as described elsewhere ( 26 ): 41BBL (Clone C65–485; BD Biosciences, San Jose, CA) conjugated with GAM-APC antibody (BD Biosciences; cat. 550826), CD80-PerCP (eBioscience, San Diego, CA; cat. 46080942); CD3-APC (clone HIT3a; cat. 555342), CD4-PECy7 (clone SK3; cat. 560909), CD8-APCH7 (clone SK1; cat. 560179), CCR7-FITC (clone 150503; cat. 561271), CD62L-APC (clone DREG-56; cat. 559772), CD95-Pacific Blue (clone DX2; cat. 562616), and CD45RO-PercP (clone UCHL1; cat. 560607) (all BD Biosciences, Mountain View, CA).

Techniques: Transduction, Expressing

Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and minicircle constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18

Journal: Cancer Cell International

Article Title: Dual-biomarkers encoding tumor-activatable minicircles with scaffold/matrix attachment region motif for sensitive and sustained blood and urine-based cancer detection

doi: 10.1186/s12935-026-04241-2

Figure Lengend Snippet: Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and minicircle constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18

Article Snippet: Parental plasmids and minicircles were produced using the MC-EasyTM Minicircle Production System (Cat# MN920A-1, System Biosciences, Mountain View, CA), following the protocols described in the previous study [ ].

Techniques: Transfection, Biomarker Discovery, Expressing, Construct, Enzyme-linked Immunosorbent Assay, Imaging, Control